Exciton dynamics in multichromophoric system visualized by fluorescence polarization imaging
by
Sumera Tubasum(PIEAS University, Islamabad and Lund University)
→
Europe/Stockholm
FA 31
FA 31
Description
Recent development in bio-inspired nanotechnology requires the essential knowledge of properties of molecular systems at the nanometer scale. The microscopy techniques, with combination of different properties of light such as wavelength, polarization and energy, are being employed to investigate molecular systems. In this contribution, a two-dimensional polarization imaging technique is developed and evaluated for the study of the photophysics of natural light harvesting pigment-protein complexes. [1] The experiment is based on the measurement of fluorescence intensity of individual complexes, by rotating the linearly polarized excitation light while the emission is detected through a rotating polarizer. The parameters of polarization study are sensitive probes to determine the spatial orientations, electronic and vibrational states, coherent or incoherent molecular interactions, symmetry of exciton states and length of the exciton coherence domain of individual complexes. The technique also provides measurement of angular energy transfer and is particularly suitable to apply to multichromophoric system.[2]
[1] S. Tubasum, I. G. Scheblykin, T. Pullerits, J. of Phys. B. 115, 4963-4970 (2011).
[2] O. Mirzov, R. Bloem, P. R. Hania, D. Thomsson, H. Lin, and I. G. Scheblykin, Small, 5, 1877-1888 (2009).