Whole genome mapping of 5' RNA ends in bacteria by tagged sequencing : A comprehensive view in Enterococcus faecalis
by
Nicolas Innocenti(KTH)
→
Europe/Stockholm
122:026
122:026
Description
Enterococcus faecalis is the third cause of nosocomial infections. To obtain the first snapshot of transcriptional organisations in this bacterium, we used a modified RNA-seq approach enabling to discriminate primary from processed 5' RNA ends. We also validated our approach by confirming known features in Escherichia coli.
In addition to numerous observations relevant to the biology of E. Faecalis, the presented data constitutes a significant a step towards the inference of regulatory processes at transcriptional and post-transcriptional levels in a comprehensive manner.
The presentation focuses on the new experimental method developed in this work, its unique capabilities as well as its drawbacks.
