Speaker
Erik Aurell
(KTH)
Description
Modern high-throughput DNA sequencing enables many
experimental approaches that address other questions than to
find the nucleotide sequences in a genome. Here I report on
one such application where we combine RNA sequencing (or
"transcriptomics") with selective tagging of RNA sequences
to map out many transcription start sites on a bacterial
genome. This is of interest as RNA molecules are typically
degraded in the living cell, and sequencing all RNA
therefore gives a mixture of the primary and the processed
fraction, and thus a mixture of transcription start sites
and processing sites. The method also gives access to other
information such as about one hundred new non-coding genes
(that do not code for a protein), and one case where one can
separate transcriptional and post-transcriptional regulation.
