Mar 26 – 28, 2014
Nordita, Stockholm
Europe/Stockholm timezone

The Primary and Processed Bacterial Transcriptome of the Human Pathogen Enterococcus Faecalis

Mar 27, 2014, 5:00 PM
45m
132:028 (Nordita, Stockholm)

132:028

Nordita, Stockholm

Speaker

Erik Aurell (KTH)

Description

Modern high-throughput DNA sequencing enables many experimental approaches that address other questions than to find the nucleotide sequences in a genome. Here I report on one such application where we combine RNA sequencing (or "transcriptomics") with selective tagging of RNA sequences to map out many transcription start sites on a bacterial genome. This is of interest as RNA molecules are typically degraded in the living cell, and sequencing all RNA therefore gives a mixture of the primary and the processed fraction, and thus a mixture of transcription start sites and processing sites. The method also gives access to other information such as about one hundred new non-coding genes (that do not code for a protein), and one case where one can separate transcriptional and post-transcriptional regulation.

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