Speaker
Asya Minervina
Description
Yellow fever vaccination is a well-established model of acute viral
infection in human. Primary immunization elicits strong T-cell response
and formation of long-lived memory. However, little data exists on T-
cell response to revaccination. We applied deep TCR-profiling to track
T-cell clones after yellow fever vaccination and revaccination. We
isolated PBMCs, CD4+, CD8+, memory and MHC-dextramer positive
subpopulations from the first-time vaccinee and revaccinated donor on
several time points before and after YFV17D immunization. TCR
repertoires were reconstructed, and expanded TCRs were identified
using edgeR. We identified 1580 expanded clonotypes in first-time
vaccinee and 204 in the revaccinated donor. YFV17D-specificity was
confirmed by sequencing of MHC-dextramer positive subpopulation. In
first-time vaccinee fraction of responded clones sharply peaked (6.1%)
at day 15 after immunization. However, in revaccinated donor fraction
of responded clones reached 0.4% by day 5 and slightly increased on
days 10 and 15. Analysis of individual clones dynamics showed the
presence of two distinct groups of clones in the revaccinated donor. T-
cell response to booster YFV17D vaccination, even 30 years after the
first, differs from response to primary immunization both by intensity
and dynamics. We detected the early expansion of some T-cells in
response to revaccination and delayed response of putatively naive T-
cells.
Primary author
Asya Minervina
