Speaker
Dr
Lukas Bogunovic
(Universität Bielefeld)
Description
We present our recent studies concerning micro-and
nanofluidic devices that are capable of detecting,
manipulating and separating single DNAs with different
lengths and conformations [1,2] and with complexed molecules
such as polymerases or chemotherapeutics [3,4].
The first device consists of a straight microchannel
structured with an array of non-conducting posts, which
create dielectrophoretic traps when a voltage is applied.
The escape process of DNA molecules migrating through the
structure can be modelled using Kramers’ rate theory and
quantitative values for DNA polarizability are extractable.
The same principle can be exploited to separate single
linear and supercoiled DNA molecules by length and
conformation within less than 4 minutes.
The central element of the second device is a 3D-structured
microfluidic channel with a bended constriction that
reduces the channel height to about 670 nm. At this barrier,
dielectrophoretic forces selectively deflect DNA with a
bound agent (proteins or antibiotics) and let the
uncomplexed DNA fragments pass unhindered. As the device
operates continuously, no actual separation time exists and
the separated samples can be immediately collected or
post-processed. The result of the separation can be observed
in real-time allowing for an on-line optimization of the
parameters of separation during operation. As a
proof-of-principle, we demonstrate the separation of DNA
molecules with different length and DNA/polymerase as well
as DNA/Actinomycin D complexes from uncomplexed DNA.
[1] J. Regtmeier, T.T. Duong, R. Eichhorn, D. Anselmetti, A.
Ros, Analytical Chemistry 79, 3925-3932 (2007)
[2] J. Regtmeier, R. Eichhorn, L. Bogunovic, A. Ros, D.
Anselmetti, Analytical Chemistry 82, 7141-7149 (2010)
[3] M. Everwand, D. Anselmetti, J. Regtmeier, Proceedings of
the µTAS
2010, 19-21 (2010)
[4] M. Everwand, R. Eichhorn, J. Regtmeier, D. Anselmetti,
in preparation
Primary author
Dr
Lukas Bogunovic
(Universität Bielefeld)