Speaker
Johan Elf
(Uppsala University)
Description
I will present our resent advancements in tracking
individual freely diffusing fluorescent
proteins molecules at in the cytoplasm of bacterial cells.
High speed tracking of individual
mEos2 molecules reveals how the physical nature of the
bacterial cytoplasm is perceived by
a protein molecule. In vivo tracking of individual fusion
proteins further makes it possible
to study intracellular kinetics high time resolution without
synchronizing the population of
molecules. For example by monitoring the ribosome binding
kinetics of the key regulatory
enzyme RelA, we have developed a single molecule assay to
study stress response and amino
acid starvation at the level of individual bacteria.
