Intracellular diffusion and kinetics at the level of single molecules

Feb 23, 2011, 3:00 PM
45m
FB52 (AlbaNova Main Building)

FB52

AlbaNova Main Building

Speaker

Johan Elf (Uppsala University)

Description

I will present our resent advancements in tracking individual freely diffusing fluorescent proteins molecules at in the cytoplasm of bacterial cells. High speed tracking of individual mEos2 molecules reveals how the physical nature of the bacterial cytoplasm is perceived by a protein molecule. In vivo tracking of individual fusion proteins further makes it possible to study intracellular kinetics high time resolution without synchronizing the population of molecules. For example by monitoring the ribosome binding kinetics of the key regulatory enzyme RelA, we have developed a single molecule assay to study stress response and amino acid starvation at the level of individual bacteria.

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