Mar 23 – 24, 2009
KTH - Royal Institute of Technology
Europe/Stockholm timezone

High Content Imaging and Analysis approach to investigation of nanoparticle/cell interactions

Mar 24, 2009, 9:15 AM
F3 (KTH - Royal Institute of Technology)


KTH - Royal Institute of Technology

Lindstedtsväg 26


Prof. Yuri Volkov (Trinity College Dublin, Ireland)


Rapid development of nanotechnology consistently increases the likelihood of human contact with environmentally presented and manufactured nanomaterials, i.e. the tiny objects ranging in size from one to several hundreds of nanometres and featuring an extreme diversity in shapes and physico-chemical properties. However, there is still very little definitive systematic information about the consequences of interactions of nano-scale objects with human cells of diverse origin and therefore safety-related issues are high on the agenda in the emerging scientific area of nanomedicine. On the other hand, optimistic expectations are associated with the opportunities of using the nanoparticles as a new class of drug delivery systems, arising from the fact that the finite, but tunable size of the engineered nanostructures used as drug delivery vehicles can impose very precise nano-scale drug distribution barriers at the level of cells, tissues and entire organism thereby eliminating undesirable side effects pertinent to most contemporary medicines. High content imaging and analysis (HCA) approach provides a unique integrated technological toolkit for visualization and physical characterization of nanoparticle-cell interactions. An exceptional aspect of this approach is that it is possible to identify individual cell, as well as population responses associated with nanoparticle exposure, as in this way subtle effects on small groups of cells within the whole, which could be averaged out by only screening the whole set, are fully registered and elucidated. We provide here an overview of HCA application scenarios for screening the safety and intracellular distribution of nanomaterials with promising biomedical application potential. Supported by the Health Research Board of Ireland, Science Foundation of Ireland SRC BioNanoInteract and EU FP-6 Consortium NanoInteract.

Primary author

Prof. Yuri Volkov (Trinity College Dublin, Ireland)

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